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Operetta CLS 高内涵系统

Operetta CLS? 系统帮助您探索常规实验与复杂创新实验中的生物学信息。通过一系列创新技术的有机结合,Operetta CLS? 同时满足精细细胞细节成像对高速、高灵敏度、高分辨率检测多方面的需求。同时,凭借功能强大的Harmony 4.5版软件,Operetta CLS? 可帮助您发现并定量细微的细胞表型改变。

部件号 HH16000000






详情 信息

Operetta CLS? 系统同时兼具高速、高灵敏度的数据采集能力,以及广受赞誉的功能强大,界面友好的数据分析解读功能。为了进一步助力您的研究工作,我们提供了从全套高内涵筛选的解决方案,从高内涵仪器到成像使用标准多?#35013;?#21040;自动化液体工作站以及后续丰富的信息学数据处理解决方案。Perkinelmer为你提供一站式的无缝连接服务,帮助您搭建全面专业高内涵筛选平台。完善的平台搭建服务与界面友?#20204;?#21151;能强大的高内涵分析软件harmony ,帮您扫除高内涵筛选路?#31995;?#21518;顾之忧。与您一路同行,快速高效的开始您的高内涵筛选工作。


Operetta CLS高内涵筛选系统以全新设计的高灵敏的光路为核心,确保最高效的样品信息的激发和检测。

  • 首开先河的高能、长寿命8色高内涵固态LED光源提供最优化、最可?#24247;?#33639;光信号采集和数据采集。
  • 专利全自动水镜系统同时满足了细胞图像采集在高分辨率、高速以及光毒性最小的需求。
  • 转盘共聚?#22266;?#20379;?#19997;?#36895;、低光毒性的共聚焦成像,在获得共聚焦质量图像的同时,提供了活细胞在线实时检测以及三维扫描检测功能。
  • 大靶免sCMOS相机提供了低噪声、宽动态范围、高分辨?#23454;?#22270;像检测,为低光毒性、高灵敏度、定量检测提供了绝佳的检测方案。
  • 用户友好的编码型滤光片,提供了灵活、特异性高、且?#23376;?#25805;作的成像体验。
  • 简单且功能强大的Harmony 4.5高内涵软件成像分析软件提供了符合生物学使用习惯、逻辑清晰、功能强大的实验设置、图像采集、图像分析以及数据存储和解读。


Operetta CLS? 满足您实验中的多种实验需求。您可根据您的实验需求定制不同模块的高内涵系统。推荐配置包括:

Operetta CLS? Quattro

  • 四色LED以及宽场荧光,Operetta CLS基础配置满足您实验室日常实验所需要的灵敏度和分辨率,同时为您提供了保留了丰富的升级空间。

Operetta CLS? FLEX

  • 八色LED,共聚焦及宽场荧光成像,FLEX 配置提供全面的、灵活的、特异性极高的荧光激发、荧光检测配置,同时该型号也提供更加高端的性能升级空间。

Operetta CLS? LIVE

  • 在FLEX型号基础上,配置活细胞气体控制与温度控制以及低光毒性、高分辨率、高荧光亮度的水镜头系统,该系统是低光毒性、高灵敏度的活细胞成像实验的理想选择。


21 CFR Part 11兼容 No
自动化兼容 Yes
检测方法 Transmission, Fluorescence
高度 45.0 cm
显像模式 High Content
接口 Ethernet
长度 98.0 cm
光源 LED
便携式 No
产品品牌名称 Operetta CLS
保修 Yes
宽度 66.0 cm
  • 所有


Face-to-Face with Cancer: The Power of High-Content Imaging and Analytics to Illuminate Cell Population Dynamics

To advance our biological understanding of cancer and improve treatment efficacy, we are utilizing quantitative high-content imaging to illuminate the dynamic interactions between cancer cells and their microenvironment.

Moving from 2D to 3D cell models: Overcoming challenges to achieving high-quality high-content results

Over recent years, more biologists have started using 3D cellular models, which provide cells with something more akin to their natural microenvironment. However, they present a number of challenges to be overcome.

Image-Based Screening for Precision Medicine and Immunotherapy

Discover how image-based screening can be performed directly in primary material, enabling more clinically translatable drug screening, and ultimately, functional precision medicine.

HCS for Predictive Toxicology and in vitro Pathology Using Simple 3D Microtissues

This webinar will describe the integration of biology and engineering to devise simple, high-throughput 3D human microtissues as predictive biology platforms that reflect human physiology and disease.



3D Cell Culture Solutions Brochure

Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.

HCS Operetta CLS 应用案例


Operetta CLS

高内涵成像筛选系统Operetta CLS





Adding Depth: 3-D Cell Culture

Download this booklet from The Scientist and PerkinElmer to learn about how the third dimension affects cell behavior, the similarities and differences between 2-D and 3-D culture, common 3-D culture models, and how to image and analyze 3-D culture models.

Cellular Analysis in 3D

While 3D cell culture provides unprecedented opportunities for both increased physiological relevance and analysis using a high-content approach, it is also more complex than traditional 2D cell culture. This booklet, from Biocompare and PerkinElmer, will unravel some of the complexities often encountered when using 3D cell models for drug discovery and provide insights and solutions that will streamline workflows and facilitate the development of effective therapeutics. Topics covered include: Reagents and instruments for growing, detecting, and analyzing 3D cell models; 3D culture methodologies; the value of high-content screening with 3D cell models and how to improve image acquisition and image analysis with high-content assays.



Analyzing ERK Signal Transduction in Live Cells Using a FRET-Based Biosensor

Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This application note describes how a live cell FRET assay to study ERK signaling was performed on the Operetta CLS? high-content analysis system. The optimized design of the FRET-based biosensor, the high-quality imaging of the Operetta CLS system and the easy-to-use image analysis tools of the Harmony? software contribute to the robustness of the high-content assay.

Automated Single Cell Tracking using the Operetta System: Analyzing Chemokinesis of Cancer Cells

In this application note, we describe a high-content screening application for analyzing the migration of non-small cell lung cancer cells in a live cell assay. Using the Operetta? high-content imaging system and digital phase contrast imaging, we tracked migrating cancer cells using automated single cell tracking in the Harmony? high-content imaging and analysis software.

Distinguishing Cell Types by Phenotypic Profiling of the Nucleus

The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties. As a result, valuable information from precious cells and disease models is not utilized. As nearly all screening approaches require a nuclear counterstain such as Hoechst to facilitate segmentation, phenotypic profiling of the nuclei can offer new and additional perspectives on assays at no extra cost.

Phenotypic Analysis of Hypertrophy in Human iPSC-Derived Cardiomyocytes

Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assay, the Opera Phenix system is 4 times faster than the previous Opera? system. Primary neuron morphology is analyzed in a straightforward approach using Harmony software. Careful assay optimization can increase throughput, and minimize the data burden, without compromising assay performance.

3D Volumetric Analysis of Luminal Spaces Inside Cysts or Organoids

High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. In this technical note we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS? high-content analysis system.

We address:

  • How to reduce the loss of image resolution as you image deeper into the sample
  • How to enable 3D segmentation and analysis for your 3D high-content assays
  • How to increase throughput for 3D imaging through shorter exposure times
  • How to eliminate long and tedious data transfer steps
  • PDF 1 MB
    3D Volumetric and Zonal Analysis of Solid Spheroids

    Multicellular 3D “oids” (tumoroids, spheroids, organoids) have the potential to better predict the effects of drug candidates during preclinical screening. However, compared to 2D cell monolayers, assays using 3D model systems are more challenging.

    In this technical note we describe how to image and analyze solid spheroids in 3D using the Opera Phenixand OperettaCLS high-content screening systems and Harmony® 4.8 imaging and analysis software.

    We address:

  • How to reduce image acquisition time and data volume
  • How to analyse fully and partially imaged spheroids in 3D
  • How to define zones and quantify spatial differences within spheroids
  • PDF 1 MB
    Clearing Strategies for 3D Spheroids

    In drug discovery programs, multicellular spheroids have emerged as powerful tools to bridge the gap between in vitro cell culture models and in vivo tissues. However, one of the greatest challenges in higher throughput 3D imaging is the acquisition of images of solid spheroids, owing to the reduced light penetration.

    One solution is to use optical clearing techniques, which can enhance the imaging depth in spheroids by removing lipid and protein molecules.

    In this technical note, we compare different optical clearing strategies for 3D spheroids and quantify the clearing effectiveness and alterations in spheroid morphology, and demonstrate how to increase imaging depth in 3D spheroids by a factor of four.

    PDF 1 MB
    How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

    Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS? and Opera Phenix? high-content systems, avoid phototoxicity with gentle digital phase contrast imaging, and analyze cell growth and morphology on a single cell level without fluorescence staining.

    PDF 3 MB
    The Benefits of Automated Water Immersion Lenses for High-Content Screening

    Balancing the key factors in HCS imaging - sensitivity, resolution and speed - can be challenging since they cannot be optimized independently: changing one impacts the others. Nevertheless, there is a way to overcome some of the obstacles and here we explain why the choice of the objective lens is critical.

    PDF 1 MB


    Five Top Tips for a Successful High-Content Screening Assay with a 3D Cell Model System

    Whether you’re familiar with high-content screening, or a newcomer, you’ll need the right tools and strategies to overcome the challenges of using complex 3D cell models in such an assay. For example, growing consistent, reproducible 3D cultures can be problematic and imaging large, thick cell samples can be challenging, while managing the huge volumes of data generated is perhaps the most demanding aspect of all. In this article, we provide our top tips for running a successful high-content screening assay using a 3D cell model. Learn how you can: Generate uniform 3D cell models, Get the best quality images, Minimize imaging time and volume of data, Get deeper insights from your 3D cell model and Avoid unnecessary data transfer steps.

    PDF 1 MB


    5 Top Tips for a Successful High-content Screening Assay with a 3D Cell Model System

    Whether you’re familiar with high-content screening and are looking to exploit the increased physiological relevance of complex 3D cell models, or you want to take your analysis of 3D cell models to the next level, migrating from simple plate-reader assays to a high-content approach, you’ll need the right tools and strategies to overcome the challenges these models present.

    PDF 1 MB


    A Workflow to Characterize and Benchmark Human Induced Pluripotent Stem Cells

    Human induced pluripotent stem cells (iPSCs) offer tremendous opportunities for disease modeling and discovery of novel therapeutics. The UK-based Human Induced Pluripotent Stem Cell Initiative (HipSci) aims to advance iPSC technology and pave the way to discovering how genomic variation impacts cellular phenotype by offering the scientific community access to a vast panel of cell lines with thorough characterization and data analysis tools. This case study details a phenotypic screen used to characterize human iPSCs on diverse extracellular matrix substrates, and a method for the capture of specific phenotypes emerging upon cell-to-cell contact.

    PDF 1 MB
    High-Content Analysis of Drug-Induced Oligodendrocyte Differentiation Promoting Remyelination in Multiple Sclerosis

    One of the greatest challenges in multiple sclerosis (MS) therapy is the halting or reversal of the failure of remyelination in the brain in order to reverse disabilities in MS patients. This case study highlights the recent work of Dr. Paul Tesar and colleagues at the Case Western Reserve University School of Medicine, which could potentially lead to such novel treatments, as it aims to control the function of stem cells in the body and thereby to help the body repair itself.

    PDF 3 MB
    High-Content Analysis: Advancing Your Knowledge to Help Combat Infectious Disease

    Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches. In our e-book, learn how high-content imaging and analysis plays a significant role in infectious disease research.

    PDF 4 MB
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